Asian Journal of Transfusion Science
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Year : 2012  |  Volume : 6  |  Issue : 1  |  Page : 53-54
Anti-Leb (Lewis) antibody in renal transplantation, emphasizing the role of transfusion medicine in organ transplantation

1 Department of Transfusion Medicine, Nizam's Institute of Medical Sciences, Hyderabad, Andhra Pradesh, India
2 Department of Nephrology, Nizam's Institute of Medical Sciences, Hyderabad, Andhra Pradesh, India

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Date of Web Publication16-Apr-2012

How to cite this article:
Shanthi B, Raju D S, Dasarath D, Sandip V S. Anti-Leb (Lewis) antibody in renal transplantation, emphasizing the role of transfusion medicine in organ transplantation. Asian J Transfus Sci 2012;6:53-4

How to cite this URL:
Shanthi B, Raju D S, Dasarath D, Sandip V S. Anti-Leb (Lewis) antibody in renal transplantation, emphasizing the role of transfusion medicine in organ transplantation. Asian J Transfus Sci [serial online] 2012 [cited 2022 Dec 8];6:53-4. Available from:


Anti-Lewis antibodies rarely cause hemolytic transfusion reactions and hemolytic disease of the newborn. But, Lewis blood group system incompatibility is proved to be significant in renal allograft rejection. [1]

Here, we discuss about a 47-year-old male, known case of chronic renal disease on hemodialysis treatment for six months. He presented with signs and symptoms of renal failure and anemia, no history of blood transfusions, and was planned for renal transplantation. Two voluntary kidney donors were tested. Patient's blood group showed discrepancy in the serum grouping, positive (1+) reaction with 'O' pooled cells. Indirect antiglobulin test (IAT) was reactive 1+. Positive cross match was noticed with both the donors. Both are HLA (Human leukocyte antigen)/lymphocyte cross match compatible.

LISS - IAT at 37°C antibody screening with commercially available three red cell panel showed positive reactions with panel II (2+) and III (2+) while negative with I panel cells [Table 1]. The suspected antibody was positive at all phases of testing. Eleven red cell identification panel was positive with homozygous Le a-b+ cells (panel 1, 5, 6, 9, 10), negative with Le a+b- cells (panel 3, 4, 8), and Le a-b-cells in the panel 2, 7 [Table 2]. However, the reaction was weak and 1+ the pattern was exactly showing Le b antibody. [2]
Table 1: Antigram, three red cell panel shows Cell II and III positive reaction

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Table 2: Antigram, eleven red cell panel done at 370C AHG phase. Includes five antigen positive and six antigen negative reagent red cell samples

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The, anti-Le b was found in the patient with ImmunoglobulinG (IgG) component, causing clinically significant incompatibility with voluntary kidney donor red cells. To remove these allo-antibodies, three cycles of plasma exchange was done on three consecutive days along with Tacrolimus, MMF (Mycophenolate mofetil), and methyl prednisolone. Antibody screenings were negative after therapeutic plasma exchange. Renal transplantation was performed with one of the live kidney donor. There is no acute allograft rejection. Patient's renal parameters improved. After six weeks, Coomb's and antibody screening were tested negative. Follow-up after six months was uneventful.

Lewis incompatibility in renal transplantation shows increased risk of both cell-mediated and humoral immune-mediated allograft rejection. HLA matching and Lewis matching are not only additive but that Lewis compatibility is the more important for transplant prognosis. [1]

Schweitzer et al. reported 15 patients with positive donor-recipient cross-match who were desensitized with plasmapheresis to permit live donor transplantation under newer maintenance immunosuppressants. [3]

Lewis, Duffy and Kidd antigens which are polymorphic and immunogenic expressed on kidney may be further studied to consider them as minor histocompatibility antigens. [4]

In conclusion, minor blood group incompatibility is not necessarily a contraindication to renal transplantation. Antibody screening, identification, titration, and evaluation of these minor blood group antibodies are mandatory before transplantation.

   References Top

1.Boratyñska M, Banasik M, Ha³oñ A, Patrza³ek D, Klinger M. Blood group Lewis alloantibodies cause antibody-mediated rejection in renal transplant recipients. Transplant Proc 2007;39:2711-4.   Back to cited text no. 1
2.Judd JW. Approaches to antibody identification. Methods in Immunohaematology. 2 nd ed., section 10. Durham: Montgomery Scientific Publications; 1994. p. 207-10.  Back to cited text no. 2
3.Schweitzer EJ, Wilson JS, Fernandez-Vina M, Fox M, Gutierrez M, Wiland A, et al. A high panel - reactive antibody rescue protocol for cross match-positive live donor kidney transplants. Transplantation 2000;70:1531-6.  Back to cited text no. 3
4.Lerut E, Van Damme B, Noizat-Pirenne F, Emonds MP, Rouger P, Vanrenterghem Y, et al. Duffy and Kidd blood group antigens: Minor histocompatibility antigens involved in renal allograft rejection? Transfusion 2007;47:28-40.  Back to cited text no. 4

Correspondence Address:
B Shanthi
Department of Transfusion Medicine, Nizam's Institute of Medical Sciences, Punjagutta, Hyderabad - 500 082, Andhra Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-6247.95058

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  [Table 1], [Table 2]



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2006 - Asian Journal of Transfusion Science | Published by Wolters Kluwer - Medknow
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