Asian Journal of Transfusion Science
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Year : 2017  |  Volume : 11  |  Issue : 2  |  Page : 188-191

The prozone effect exerted by the complement-binding anti-Lea on anti-D

1 Director, Lok Samarpan Raktadan Kendra, Surat, Gujarat, India
2 Department of Neonatology, Amruta Hospital, Surat, Gujarat, India

Correspondence Address:
Sanmukh R Joshi
Lok Samarpan Raktadan Kendra, Mini Bazaar, Varachha Road, Surat - 395 006, Gujarat
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-6247.214328

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Background: Prozone phenomenon is seen with very high-titer antibodies in an immune serum. Aim: The prozone effect on anti-D by a low-titer anti-Lea was investigated associated with neonatal jaundice. Materials and Methods: Standard methods were used in investigations. Results: The child was born at full-term developed mild jaundice. With weak direct antiglobulin test+, her indirect serum bilirubin was progressed to 27.5 mg/dL in 48 h. Anti-D and anti-Lea were detected in the mother. Both these antibodies were detected in the child's serum though the eluate from red blood cells (RBCs) contained only anti-D. Mother's anti-D was masked by anti-Lea if the RBCs possessed both the antigens together. Anti-D was revealed only with D-positive RBCs lacking Lea or if the serum was modified by mixing with Lea+ saliva or was heated at 56°C or fortified with citrate solution. Conclusion: An anti-D showed prozone effect exerted by the complement-fixing anti-Lea in the test.

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2006 - Asian Journal of Transfusion Science | Published by Wolters Kluwer - Medknow
Online since 10th November, 2006