| CASE REPORT |
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| Year : 2020 | Volume
: 14
| Issue : 1 | Page : 79-82 |
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Implication of a positive virtual crossmatch with negative flow crossmatch: A mind-boggler
Mohit Chowdhry, Soma Agrawal, Yogita Thakur, Sandeep Guleria, Vandana Sharma
Department of Transfusion Medicine, Molecular Biology and Transplant Immunology, Indraprastha Apollo Hospital, New Delhi, India
Correspondence Address:
Dr. Soma Agrawal
Department of Transfusion Medicine and Transplant Immunology, Indraprastha Apollo Hospital, New Delhi - 110 076
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ajts.AJTS_159_18
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There are occasions when tests performed before considering a patient for transplant are ambiguous and require further workup. One such condition is the presence of a positive virtual crossmatch (VXm) (anti-human leukocyte antigen [HLA-A]*26: 01 antibody in this case) with a negative complement-dependent cytotoxicity, Luminex, and flow crossmatch. To ascertain the nature of the antibody, the beads used in single-antigen bead assay (SAB) were treated by acid to denature the antigens and retested with the control and test sample. The mean fluorescence intensities (MFIs) from the patient sera with acid-treated beads increased considerably as compared to the regularly untreated SAB indicating additional antigen epitopes become available by the denaturation process. The MFIs of the antibodies from that of the control sera were reduced to half on testing with the acid-treated SAB assay, indicating that HLA antigen HLA-A*26 was susceptible to acid treatment. Therefore, results of VXm should be interpreted with caution. |
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