Asian Journal of Transfusion Science
Home About Journal Editorial Board Search Current Issue Ahead of print Back Issues Instructions Subscribe Login  Users: 314 Print this page  Email this page Small font sizeDefault font sizeIncrease font size 


 
ILLUSTRATION Table of Contents   
Year : 2020  |  Volume : 14  |  Issue : 1  |  Page : 98
Validation of lateral flow assay for blood grouping on hemolyzed sample


Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India

Click here for correspondence address and email

Date of Submission27-Feb-2017
Date of Acceptance06-Dec-2017
Date of Web Publication24-Jul-2020
 

How to cite this article:
Chenna D, Shastry S, Kanukula VR, Baliga B P. Validation of lateral flow assay for blood grouping on hemolyzed sample. Asian J Transfus Sci 2020;14:98

How to cite this URL:
Chenna D, Shastry S, Kanukula VR, Baliga B P. Validation of lateral flow assay for blood grouping on hemolyzed sample. Asian J Transfus Sci [serial online] 2020 [cited 2020 Oct 22];14:98. Available from: https://www.ajts.org/text.asp?2020/14/1/98/290637




Hemolysis, due to the breakdown of red blood cells, can occurin vivo or in vitro.[1] Grossly, hemolyzed samples can affect the results of many tests, and hence is a rejection criterion in most of the laboratories.In vitro causes can be addressed by repeat sampling; however, on some rare occasions performing a test on hemolyzed samples is inevitable. Occasionally, we receive hemolyzed samples from postmortem cases, following fetal autopsy for blood grouping at our immunohematology laboratory. Reporting blood group on the hemolyzed sample is difficult using current techniques (tube test and column agglutination technology), and most of the time, it goes unreported with a comment on sample suitability [Figure 1]a and [Figure 1]b.
Figure 1: Blood grouping on hemolyzed sample. (a) Blood grouping by tube technique before and after hemolysis of the sample, (b) Column agglutination technique, (c) Lateral flow technique

Click here to view


Hence, we tried blood grouping of hemolyzed blood samples, using grouping cards based on the principle of lateral flow guided by capillary action (Erycard, Tulip Diagnostics, Verna, Goa). Sample pad with predried monoclonal antibodies (anti-A, anti-B, and anti-D) and a negative control slot (without reagent) is used in the card. The test is performed using whole-blood sample and reagent buffer as per manufacturer instructions. Visible red color patch indicates the presence of antigen and no color its absence.

To validate this procedure, we selected 20 blood samples of different combination of blood groups. The samples were subjected to hemolysis by adding distilled water or by freeze-thawing. Blood grouping was done by both conventional (test tube and column agglutination) technique and the proposed technique before and after hemolysis of the samples. Two investigators were involved in the process to avoid the interpretation bias.

The results of hemolyzed sample obtained using cards were accurately matching with the prehemolyzed sample results [Figure 1]c. Hence, we recommend the use of cards based on the principle of lateral flow technique for blood group reporting in the hemolyzed sample.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Lippi G, Blanckaert N, Bonini P, Green S, Kitchen S, Palicka V, et al. Haemolysis: An overview of the leading cause of unsuitable specimens in clinical laboratories. Clin Chem Lab Med 2008;46:764-72.  Back to cited text no. 1
    

Top
Correspondence Address:
Dr. Shamee Shastry
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ajts.AJTS_28_17

Rights and Permissions


    Figures

  [Figure 1]



 

Top
 
  Search

  
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
    Email Alert *
    Add to My List *
* Registration required (free)  


    References
    Article Figures

 Article Access Statistics
    Viewed186    
    Printed12    
    Emailed0    
    PDF Downloaded0    
    Comments [Add]    

Recommend this journal

Association Contact us | Sitemap | Advertise | What's New | Feedback | Copyright and Disclaimer

2006 - Asian Journal of Transfusion Science | Published by Wolters Kluwer - Medknow
Online since 10th November, 2006