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| Year : 2020 | Volume
: 14
| Issue : 1 | Page : 98 |
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| Validation of lateral flow assay for blood grouping on hemolyzed sample |
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Deepika Chenna, Shamee Shastry, Vijayram Reddy Kanukula, B Poornima Baliga
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India
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| Date of Submission | 27-Feb-2017 |
| Date of Acceptance | 06-Dec-2017 |
| Date of Web Publication | 24-Jul-2020 |
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How to cite this article:
Chenna D, Shastry S, Kanukula VR, Baliga B P. Validation of lateral flow assay for blood grouping on hemolyzed sample. Asian J Transfus Sci 2020;14:98 |
How to cite this URL:
Chenna D, Shastry S, Kanukula VR, Baliga B P. Validation of lateral flow assay for blood grouping on hemolyzed sample. Asian J Transfus Sci [serial online] 2020 [cited 2020 Oct 22];14:98. Available from: https://www.ajts.org/text.asp?2020/14/1/98/290637 |
Hemolysis, due to the breakdown of red blood cells, can occurin vivo or in vitro.[1] Grossly, hemolyzed samples can affect the results of many tests, and hence is a rejection criterion in most of the laboratories.In vitro causes can be addressed by repeat sampling; however, on some rare occasions performing a test on hemolyzed samples is inevitable. Occasionally, we receive hemolyzed samples from postmortem cases, following fetal autopsy for blood grouping at our immunohematology laboratory. Reporting blood group on the hemolyzed sample is difficult using current techniques (tube test and column agglutination technology), and most of the time, it goes unreported with a comment on sample suitability [Figure 1]a and [Figure 1]b. | Figure 1: Blood grouping on hemolyzed sample. (a) Blood grouping by tube technique before and after hemolysis of the sample, (b) Column agglutination technique, (c) Lateral flow technique
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Hence, we tried blood grouping of hemolyzed blood samples, using grouping cards based on the principle of lateral flow guided by capillary action (Erycard, Tulip Diagnostics, Verna, Goa). Sample pad with predried monoclonal antibodies (anti-A, anti-B, and anti-D) and a negative control slot (without reagent) is used in the card. The test is performed using whole-blood sample and reagent buffer as per manufacturer instructions. Visible red color patch indicates the presence of antigen and no color its absence.
To validate this procedure, we selected 20 blood samples of different combination of blood groups. The samples were subjected to hemolysis by adding distilled water or by freeze-thawing. Blood grouping was done by both conventional (test tube and column agglutination) technique and the proposed technique before and after hemolysis of the samples. Two investigators were involved in the process to avoid the interpretation bias.
The results of hemolyzed sample obtained using cards were accurately matching with the prehemolyzed sample results [Figure 1]c. Hence, we recommend the use of cards based on the principle of lateral flow technique for blood group reporting in the hemolyzed sample.
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
 References | |  |
| 1. | Lippi G, Blanckaert N, Bonini P, Green S, Kitchen S, Palicka V, et al. Haemolysis: An overview of the leading cause of unsuitable specimens in clinical laboratories. Clin Chem Lab Med 2008;46:764-72.  |
Correspondence Address:
Dr. Shamee Shastry
Department of Immunohematology and Blood Transfusion, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ajts.AJTS_28_17
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