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  Indian J Med Microbiol
 

Figure 1: Representative results of a multiplex PCR analysis in our study. A 3% agarose gel of the PCR products obtained with genomic DNAs from a D+sample, a D− sample resulting from RHD gene deletion and different RHD variants. Lane 1: DNA marker; lane 2: Weak D type 4.0; lane 3: D+; lane 4: Variant (RHD exons 4 and 5 negatives); lane 5: Variant (only RHD exon 9+); lane 6, D− (RHD exons 4 and 5 negatives); lane 7: D−. C: Human growth hormone gene, which generating a 429 pb fragment, was used at internel control

Figure 1: Representative results of a multiplex PCR analysis in our study. A 3% agarose gel of the PCR products obtained with genomic DNAs from a D+sample, a D− sample resulting from <i>RHD</i> gene deletion and different <i>RHD</i> variants. Lane 1: DNA marker; lane 2: Weak D type 4.0; lane 3: D+; lane 4: Variant (<i>RHD</i> exons 4 and 5 negatives); lane 5: Variant (only <i>RHD</i> exon 9+); lane 6, D− (<i>RHD</i> exons 4 and 5 negatives); lane 7: D−. C: Human growth hormone gene, which generating a 429 pb fragment, was used at internel control